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Nina Alayne Dathan

Curriculum

Nina Dathan’s scientific experience encompasses a wide range of molecular, cellular and biochemical techniques contributing to an equally  vast range of projects. Initially working with numerous RNA and DNA techniques, her  interest has gradually shifted to working more with proteins .  She has applied her extensive basic knowledge to the optimization of expression and purification of an enormous range of recombinant proteins in E.coli, mainly of biomedical interest, as well as their biochemical and structural characterization. In fact for a number of years she has been responsible for running an expression and purification service for such proteins in and around the Naples area.
She is and has been involved in numerous national and international collaborations, is author to over 40 international scientific publications and has contributed to various meetings, both in Italy and abroad.
Having recently joined Dr. Alberto Luini’s group she will apply her technical abilities towards helping understand signaling and trafficking in the mammalian golgi complex.  Currently she is working on a series of  constructs to block transport of cargo across maturing cisternae.

1981-1982:Undergraduate student, ICRF, London,

1983-1984:Technician LMB, Cambridge,UK,  John Gurdon’s lab.

1984-1985: Researchexperience Institut Jaques Monod, Jussieu, Paris, Marcel Mechali’s  lab.

1985: Research Assistant, Celltech, Slough, UK

1986-1991: Research Assistant, EMBL, Heidelberg, Germany, Iain Mattaj’s Lab.

1992-1996: Researcher Cellular and Molecular Biology and Pathology lab., University of Naples “Federico II” Prof. Giancarlo Vecchio’s lab.

1997-2001: CNR Technologist,(art.23) Biocrystallography Centre, Naples, Prof. Carlo Pedone’s lab.

1997-2000: Guest  at Zoological Station “Anton Dohrn”, Prof. Roberto Di Lauro’s lab.

1999: Guest at NIH, Bethesda, USA, Dr. Ira Paston’s lab.

2001-2013: CNR Technologist,(permanent position) Institute of Biostructures and Bioimaging, Naples, Prof. Carlo Pedone’s lab.

2002-2007: Scientific coordinator Recombinant Protein group, BioGeM, Campania

2013-now: CNR Technologist, Institute of Protein Biochemistry, Dr. Daniela Corda’s group

2014-2015: Guest at Imperial College, London, UK – Prof. Graham William’s group

Key Publications

Bellelli R, Castellone MD, Guida T, Limongello R, Dathan  NA, Merolla  F, Cirafici AM, Affuso A, Masai  H, Costanzo  V, Grieco  D, Fusco  A, Santoro M, Carlomagno  F.   NCOA4 transcriptional coactivator inhibits activation of DNA replication origins.  Molecular Cell,  2014  Jul 3; 55: 1-15                  

D'ambrosio K, Lopez M, Dathan NA, Ouahrani-Bettache S, Köhler S, Ascione G, Monti SM, Winum JY, De Simone G. Structural basis for the rational design of new anti-Brucella agents: the crystal structure of the C366S mutant of L-histidinol dehydrogenase from Brucella suis.Biochimie. 2014 Feb;97:114-20.

Dathan NA, Alterio V, Troiano E, Vullo D, Ludwig M, De Simone G, Supuran CT, Monti SM.  Biochemical characterization of the chloroplastic β-carbonic anhydrase from Flaveria bidentis (L.) "Kuntze".  J Enzyme Inhib Med Chem. 2013 Jul 29. 

Amendola E, Sanges R, Galvan A, Dathan N, Manenti G, Ferrandino G, Alvino FM, Di Palma T, Scarfò M, Zannini M, Dragani TA, De Felice M, Di Lauro R. A locus on mouse chromosome 2 is involved in susceptibility to congenital hypothyroidism and contains an essential gene expressed in thyroid.  Endocrinology. 2010 Apr;151(4):1948-58.

Viparelli F, Cassese A, Doti N, Paturzo F, Marasco D, Dathan NA, Monti SM, Basile G, Ungaro P, Sabatella M, Miele C, Teperino R, Consiglio E, Pedone C, Beguinot F, Formisano P, Ruvo M,  Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cellsJ Biol Chem. 2008 Aug 1;283(31):21769-78.

Brancaccio A, Minichiello A, Grachtchouk M, Antonimi D, Sheng H, Parlato R, Dathan N, Dlugosz AA, Missero C. Requirement of the forkhead gene Foxe1,  a target of sonic hedgehog signalling, in hair follicle morphogenesis. Hum. Mol. Genet. 2004 Nov.1; 13 (21), 2595-2606

Dathan N, Zaccaro L, Esposito S, Isernia C, Omichinski JG, Riccio A, Pedone C, Di Blasio B, Fattorusso R, Pedone PV.  The Arabidopsis SUPERMAN protein is able to specifically bind DNA through its single Cys2-His2 zinc finger motif.  Nucleic Acids Res. 2002 Nov 15;30(22):4945-51. 

Cosma MP, Panariello L, Quadro L, Dathan NA, Fattoruso O, Colantuoni V.  A mutation in the RET proto-oncogene in Hirschsprung's disease affects the tyrosine kinase activity associated with multiple endocrine neoplasia type 2A and 2B.  Biochem J. 1996 Mar 1;314 ( Pt 2):397-400.

Santoro M, Carlomagno F, Romano A, Bottaro DP, Dathan NA, Grieco M, Fusco A, Vecchio G, Matoskova B, Kraus MH, Di Fiore,PP  Activation of RET as a dominant transforming gene by germline mutations of MEN2A and MEN2B.  Science. 1995 Jan 20;267(5196):381-3.

Royer HD, Freyaldenhoven MP, Napierski I, Spitkovsky DD, Bauknecht T, Dathan N.  Delineation of human papillomavirus type 18 enhancer binding proteins: the intracellular distribution of a novel octamer binding protein p92 is cell cycle regulated.  Nucleic Acids Res. 1991 May 11;19(9):2363-71.

Scherly D, Dathan NA, Boelens W, van Venrooij WJ, Mattaj IW.  The U2B'' RNP motif as a site of protein-protein interaction.  EMBO J. 1990 Nov;9(11):3675-81.

Hamm J, Dathan NA, Mattaj IW.  Functional analysis of mutant Xenopus U2 snRNAs.  Cell. 1989 Oct 6;59(1):159-69.

Mattaj IW, Dathan NA, Parry HD, Carbon P, Krol A.  Changing the RNA polymerase specificity of U snRNA gene promoters.  Cell. 1988 Nov 4;55(3):435-42.

Taylor MV, Gusse M, Evan GI, Dathan N, Mechali M.  Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell divisionEMBO J. 1986 Dec 20;5(13):3563-70.

Mohun TJ, Brennan S, Dathan N, Fairman S, Gurdon JB.  Cell type-specific activation of actin genes in the early amphibian embryo.  Nature. 1984 Oct 25-31;311(5988):716-21.